Composite

Part:BBa_M31541:Design

Designed by: Matt Gethers   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-28)


This is the M13K07 genome refactored between the Hap1 and Bam1 Sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4031
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When removed from its place in the genome that has been determined by evolution, we can't be sure the change will be tolerated. There may be cross-talk between copied and separated elements with significant homology. Because we have removed and maintained entire portions of genes in the formation of separate genomic entities, there is also the possibility of expression of these 'partial' genes. This abstraction is useful for analysis of complex functions, but there are still interactions at the base pair level that need to be resolved before it can be implemented.


Source

Though these genes exist in nature in M13K07, they do not yet exist in this refactored form.

References